Gas chromatography is an analytical instrument, that separates compounds, in a solution, by their boiling points. The lower boiling compounds will elute out first while the higher boiling compounds will elute out second, third and fourth respectively. Not every analyte can be analyzed using GC. If the boiling point is out of the range of the instrument or the analyte cannot be vaporized, a different instrument or derivatize the sample for analysis.
AFoMs are Analytical Figures of Merit. These values characterize the performance of the instrument and the method or system, to be comparable to other instruments and methods or systems.
Purpose: To compare the Buck Scientific 810 GC to the Vernier Mini GC to analyze the variability of the different instruments.
The two analytes that were analyzed were ethyl acetate and isoamyl acetate.
Ethyl acetate is an organic compound that is a clear and colorless liquid with a sweet smell (pear drops smell). It is found in many consumer products such as glues, fingernail polish removers and cigarettes. Ethyl acetate is the ester of ethanol and acetic acid. It is manufactured on a large scale to be used as a solvent.
Isoamyl acetate is an organic compound that is a clear and colorless liquid with a sweet smell (banana flavor smell). It is slightly soluble in water, but very soluble in organic solvents. Isoamyl acetate is used to flavor oils and mixtures to flavor different foods and drinks.
Our two groups used these analytes because the analytes were similar enough to test the resolution and also because they were very easy to work with, meaning toxic levels were low and the solutions did not have to be made, just mixed together.